Changes in the dynamics of germination and growth of flax, garden cress and radish seeds under the increasing dilutions of chosen auxins.
Malarczyk E, Wojtkowiak D.
Abstract
Introduction: As it has been proved earlier the activity of the enzymes from a
phenoloxidase group of bacteria and fungi [1-5] change in accordance’s with a sine
curve as a result of the incubation with the increasing dilutions of their phenol
effectors. This phenomenon seems to be common, however it requires a greater
amount of comparative studies with the use of other kind of biological material and
its biological effectors.
Aim: To test the action of subsequent dilutions of three auxins, IAA, NAA and 2,4-D
within the range of dilution ranging from C1 (100-1) to C30 (100-30) on the chosen
plants.
Methods: Selected flax, garden cress and radish seeds were subject to germination
in the presence of solutions of the mentioned auxins in the subsequent dilutions
ranging from C1 to C30. The series of dilutions were prepared laboratory. The
cultures were carried out in the Petri dishes in water environment or in deeper plastic
vessels on the sieved soil. The assessed parameters were the length of sprouts, a
percentage of germinated seeds and a speed of plants growth.
Results: Among the three kinds of tested plants, a greater differentiation of growth
depending on the used dilution and a kind of auxin, had the flax seeds. A certain
correlation between a kind of plant and the effectiveness of a particular auxin has
been noticed. The smallest differentiation of the speed of growth was observed in
case of a garden cress seeds. The third kind of plant, a radish had an irregular rising
not only with regard to a fast increase in a plant mass but also to a kind of auxin.
Conclusion: The obtained studies proved sinusoidal relativities of growth with regard
to a plants reaction to different dilutions of a growth effector’s similarly as it was
proved on a fungal and bacterial material. Apart from that they proved a rule that the
changes in the amplitude of these transformations are dependent on the affinity
between the effectors and the examined biological object.