Fungus Metarhizium anisopliae in vitro
Posted: 08 Nov 2014, 23:02
IN VITRO ASSAY OF HOMEOPATHIC PRODUCTS AGAINST Metarhizium anisopliae (METSCH.) SOROK. (ASCOMYCOTA: CLAVICIPITACEAE)
S Damin; L FA Alves; TM Alexandre; AK Bonini
ABSTRACT: This study aimed to evaluate the in vitro effect of homeopathic Arsenicum album 24CH, Calcarea carbonica 30CH, Kali iodatum 100CH; Phosphorus 3CH, Silicea 30CH; Staphysagria 6, 30 and 100CH; Spodoptera frugiperda 30CH; Sulphur 100 and 200CH and Thuya occidentalis 200CH (applied at a concentration of 0.1% homeopathic / sterile distilled water) on the fungus Metarhizium anisopliae. All products were sprayed on the fungus previously inoculated in the potato dextrose agar (PDA) culture medium in Petri dishes, evaluating germination, the colony forming units (CFU), vegetative growth, conidial production and insecticidal activity of fungus against larvae of Diatraea saccharalis (Lepidoptera: Crambidae). All treatments were considered compatible to the fungus M. anisopliae, and did not affect the insecticidal activity of the fungus on D. saccharalis.
Keywords: Compatibility, entomopathogenic fungi, dynamized product.
S Damin; L FA Alves; TM Alexandre; AK Bonini
ABSTRACT: This study aimed to evaluate the in vitro effect of homeopathic Arsenicum album 24CH, Calcarea carbonica 30CH, Kali iodatum 100CH; Phosphorus 3CH, Silicea 30CH; Staphysagria 6, 30 and 100CH; Spodoptera frugiperda 30CH; Sulphur 100 and 200CH and Thuya occidentalis 200CH (applied at a concentration of 0.1% homeopathic / sterile distilled water) on the fungus Metarhizium anisopliae. All products were sprayed on the fungus previously inoculated in the potato dextrose agar (PDA) culture medium in Petri dishes, evaluating germination, the colony forming units (CFU), vegetative growth, conidial production and insecticidal activity of fungus against larvae of Diatraea saccharalis (Lepidoptera: Crambidae). All treatments were considered compatible to the fungus M. anisopliae, and did not affect the insecticidal activity of the fungus on D. saccharalis.
Keywords: Compatibility, entomopathogenic fungi, dynamized product.