Calendula and Arnica on rice
Posted: 21 Apr 2023, 14:06
The Influence of Ultra-High Diluted Compounds on the Growth and the Metabolites of Oriza sativa L.
Hosseinian, S. Christiane Maute, Fariba Rahimi, Cornellia Maute, Maryam Hamedi, Fateme Mirzajani ,
International Journal of High Dilution Research. Vol. 19 No. 1-2 (2020)
Abstract
Background: The ultra-high dilutions (UHDs) have been widely used in the field of human, animal and plant treatment. In the present research, the effects of the potentized ultra-high dilutions (UHDs) on physiological and biochemical variations in Oryza sativa L. (rice) were investigated.
Methods: To study the effect of UHDs (Calendula officinalis Calen. and Arnica montana Arn.) on the physiological and biochemical variations of the Oryza sativa L. (rice), 28 experiments were designed and statistically analyzed using the Design Expert 7.0.1 software over the general factorial design methodology. Three qualitative factors were studied including the UHDs/placebo usage, sterile/non- sterile experimental condition and the type and timing of the UHDs usage. The validated analysis was subjected to more extended studies on the variations in physiological growth, carbohydrate, protein content, pigment production, and amino acid patterns. To evaluate the effects of UHDs on rice, a desirable response percentage was formed from a number of healthy seedling productions, and the height percentage of the aerial parts and main roots were studied.
Results: The statistical analysis resulted in a prediction model which was more than 97% correlates with experimental results. The results showed that the UHDs increased the pH variations, carbohydrate, protein and pigment levels each by ~2.5, ~1.5, ~1.4, and ~1.4 folds, respectively. Also compared to placebo, the amount and proportion of amino acids has significantly varied, showing a statistical effect on the germination and seedling growth of the rice, as well as the stress conditions caused by the sterilization process, seedlings entrance into the light and their transition into hydroponic culture medium.
Conclusion: The use of UHDs leads to an increase in the production of chlorophyll, as well as carbohydrate and protein content. Moreover, it causes significant variations in the amino acid profile and the production of amino acids along with the photosynthesis, germination, and metabolism processes.
Keywords: Amino acid, GC/MS, Rice, Physiological parameters, Ultra-High Dilution and Potentized compounds
Hosseinian, S. Christiane Maute, Fariba Rahimi, Cornellia Maute, Maryam Hamedi, Fateme Mirzajani ,
International Journal of High Dilution Research. Vol. 19 No. 1-2 (2020)
Abstract
Background: The ultra-high dilutions (UHDs) have been widely used in the field of human, animal and plant treatment. In the present research, the effects of the potentized ultra-high dilutions (UHDs) on physiological and biochemical variations in Oryza sativa L. (rice) were investigated.
Methods: To study the effect of UHDs (Calendula officinalis Calen. and Arnica montana Arn.) on the physiological and biochemical variations of the Oryza sativa L. (rice), 28 experiments were designed and statistically analyzed using the Design Expert 7.0.1 software over the general factorial design methodology. Three qualitative factors were studied including the UHDs/placebo usage, sterile/non- sterile experimental condition and the type and timing of the UHDs usage. The validated analysis was subjected to more extended studies on the variations in physiological growth, carbohydrate, protein content, pigment production, and amino acid patterns. To evaluate the effects of UHDs on rice, a desirable response percentage was formed from a number of healthy seedling productions, and the height percentage of the aerial parts and main roots were studied.
Results: The statistical analysis resulted in a prediction model which was more than 97% correlates with experimental results. The results showed that the UHDs increased the pH variations, carbohydrate, protein and pigment levels each by ~2.5, ~1.5, ~1.4, and ~1.4 folds, respectively. Also compared to placebo, the amount and proportion of amino acids has significantly varied, showing a statistical effect on the germination and seedling growth of the rice, as well as the stress conditions caused by the sterilization process, seedlings entrance into the light and their transition into hydroponic culture medium.
Conclusion: The use of UHDs leads to an increase in the production of chlorophyll, as well as carbohydrate and protein content. Moreover, it causes significant variations in the amino acid profile and the production of amino acids along with the photosynthesis, germination, and metabolism processes.
Keywords: Amino acid, GC/MS, Rice, Physiological parameters, Ultra-High Dilution and Potentized compounds