DEVELOPING AN APPLICATION OF RAMAN SPECTROSCOPY IN HOMOEOPATHIC PHARMACEUTICAL ANALYSIS OF BARYTA MURIATICUM
Niranjan B. Joshi, Tejas P. Gosavi, Manisha P. Gajendragadkar, Amit D. Kandhare, Tanveer A. Khan
Background: Raman Spectroscopy is very beneficial and effective tool for qualitative and quantitative analysis of various pharmaceutical products or formulations. This helps in scientific validation and accurate standardization of numerous drug products. Homoeopathic medicines are conventionally prepared from various complex organic and simple inorganic substances. Trituration and succussion are two methods of preparation of homeopathic medicines. Following certain steps of mathematical dilution, it crosses even subatomic level and practically becomes undetectable on even recent cutting-edge instruments as well. In this study homoeopathic formulation of Baryta Muriaticum is specifically chosen at lower potency to establish the primary standards for homoeopathic medicines quality control through the vibrational Raman Spectroscopy.
Objective: The primary objective of this study was to perform Raman spectroscopic evaluation of homoeopathic preparations of‘Baryta Muriaticum’ (barium chloride) and its various potencies namely (3X, 6X and 12X) for pharmaceutical quality control.
Methodology: The homoeopathic formulations of Baryta Muriaticum viz., 3X, 6X and 12X were prepared according to guidelines set by Indian Homeopathic Pharmacopeia, which were further analysed by Micro Raman spectroscope to assess the Scattering of molecule at different wavelengths along with intensities at [(UGC-DAE, CSR) Raman Laboratory (DAVV), Indore (Madhya Pradesh), India]. The spectra were collected within the wavenumber region of (50 - 4000 cm-1) and analysed after suitable baseline correction.
Results: Significant structural alterations were seen in Raman spectra for homoeopathic preparations of Baryta Muriaticum 3X, 6X and 12X when compared to the vehicle control of lactose (sachrum lactis). This confirms the presence of Baryta Muriaticum in the given sample. Using linear regression model for goodness of fit, the (r2) values [3X (r2 = 0.6), 6X (r2= 0.6), and 12X (r2=0.8)], indicate that Raman spectroscopy was effective tool in detecting the variability in Raman shift (cm-1) when plotted against intensity (a.u.) which in turn possibly differentiated those three triturations of Baryta Muriaticum.
Conclusion: This study concluded that Raman vibrational spectroscopy is a sensitive and effective application in detecting variability among various homoeopathic trituarations of Baryta Muriaticum and thus helpful technique in standardization and validation of homoeopathic medicines.