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In house validation of biocrystallisation

Posted: 14 Nov 2014, 08:55
by Mark
Development, in-house validation and application of holistic process Biokristallisation for the Differentiation of wheat, carrot and apple samples of different crops and processing steps

Presented by Dr. Bernhard Johannes Kahl

foreword (Google translated!)
1988 my biography of Ehrenfried Pfeiffer was given to high school graduation, the
Nestor the research process for the Biokristallisation. I was by this
Method and the manner how it discovered and developed by Pfeiffer
was fascinated. During my research work at the Max Planck
Institute for Chemical Ecology in Jena, I remembered the
Biokristallisation and tried to knowledge about the state of research
this method or experiences with him. First, I got in touch with Dr.
Ursula Balzer-Graf in Wetzikon / Switzerland, which, inter alia, with publications
the successful differentiation between samples from different
Had emerged cropping systems. More suggestions gave me
then the work of Jens-Otto Andersen of the Agricultural
Faculty of the University of Copenhagen / Denmark, whose results he on the
IFOAM Conference had introduced in 1996. His work has meant so far
a significant step forward, as he described his method in detail and
has started in addition also to characterize it. He also installed
a computer-assisted image analysis, which opened up the possibility also
large numbers of images in a short time and people to independently evaluate
My research goal was that for a general recognition
Biokristallisation and thus for their application outside
anthroposophical circles to create the essential prerequisite
namely, to validate this method. In connection with my
PhD at the Institute for Spectrochemistry and Applied Spectroscopy in
Dortmund I had with Dr. Stavros Kromidas, then NOVIA GmbH
Saarbrücken, a specialist for the validation of laboratory methods know
learned. With him, I now advised the possibility of a holistic
Validate method. About my approaches I discussed also with
Researchers in the field of organic farming. For the validation of the
Biokristallisation I worked together with Dr. Nicolaas Busscher a
Concept and a research plan. These we laid Prof. Dr. Angelika
Ploeger before, then FH Fulda. She had already in 1988 with Prof. Dr. Hartmut
Vogtmann the basic work to holistic methods and their use published in the provision of food quality. with the
common research objective in developing the Biokristallisation to
Practice maturity, Prof. Ploeger transferred in late 2001 Nicolaas Busscher and me
the establishment and management of the working group 'Ecological
Food Quality 'at its newly built art for' Ecological
Food Quality and Food Culture 'at the University of Kassel. at the
Witzenhausen were us according to requirements
equipped chemical laboratory and two identical crystallization chambers for
Provided. As a staff, we were able savvy lab assistant
Gaby Mergardt win, in the course of this project, 21,500 crystal images
has made.
At the same time, we established a network ('Triangle') with two other
Laboratories, which also set the validation of the method for target
have: with the Louis Bolk Institute in-Driebergen / Netherlands (Machteld Huber,
Paul Doesburg) and the Laboratory of Biodynamic Research Centre in
Were / Denmark (Dr. Jens-Otto Andersen, Marianne Paulsen). by the
Promotion of private and public donors (including the Software AG
Foundation, Darmstadt and the Federal Ministry of Consumer Protection,
Food and Agriculture BMVEL or the Federal Research Centre for
Agriculture and Food, Bonn) was from 2002 a series of holistic
and analytical procedures and methods for the differentiation of samples
different origins are validated. These included u. A.
Development and application of methods for the determination of secondary
Plant materials, as part of two dissertations (2002- Andreas Werries
2005 Marco Roose were validated as of 2005). Marco Desche examined
the practicality of the electrochemical methods (thesis 2004).
Important contributions in this research project provide two of
us excited and supervised dissertations: 2003 began Aneta Zalecka their
Work on the climbing image method, which characterizes the network 'Triangle'
was, and in 2004 followed Aumaporn Meelursarn with the work on the
Optimization and application of statistical models for analyzing the images.
For the statistical analysis we were greeted by Bertram Schäfer and Boris
Kulig advised by the company Statcon in Witzenhausen.
Initially had the documentation of the procedures for the Biokristallisation
created and the evaluation can be optimized before the actual validation
could begin. Andreas Deger of the company Papyrus in Rose Garden
Watched the appropriately prepared documents in the Nicolaas
Busscher developed a computer-based laboratory documentation.
The standardization of the visual image analysis documented Sebastian
Kretschmer in a thesis (2003), where we our on the experience
Mrs Monika Röger in the sensory analysis of food
could draw. Kristine Kopp evaluated in her thesis (2005), the
published research from an ecological characterization
Food with a view to the requirements of production, trade and
This habilitation thesis was only possible because we from different institutes
defined sample material were made available. These included the
Research Institute of Organic Agriculture in Frick / Switzerland (Christine
Arncken, Dr. Paul Mäder, Dr. Franco Weibel) and the Department of Organic
Agriculture and Agronomy of the University of Kassel (Prof. Dr. Peter von Fragstein
and Niemsdorff, Michael Fleck). The samples were prepared for us by the Institute for
organic farming, the Federal Agricultural Research Centre in
Trenthorst (Prof. Dr. G. Rahmann, C. Ludwig) encoded and transported. thence
the samples were directly organized by the producer.
Since 2005 there has been a research exchange with the Institute for
Reference materials and methods of the European Union in Geel / Belgium
(Prof. Dr. Elke Anklam) and the Agricultural Faculty of the University
Pisa / Italy (Maria Kokornaczyk), where the Biokristallisation validated or
is used.
From here NOTIFIED shows how much stimulation and support I
learned for the research project from different angles. Allen
persons and institutions mentioned I would like to thank warmly. for
critical reading of my work, I thank Prof. Angelika Ploeger, Prof.
Heinz twenties / FH Merseburg, Benigna von Krusenstjern / Göttingen,
Machteld Huber, Nicolaas Busscher, Jens-Otto Andersen and Christian
Krutzinna / Witzenhausen. Further thanks also go to the staff and
Colleagues of the Department of "Organic Food Quality and
Food Culture "for the discussions and exchange of experience. particularly
I would like to thank Prof. Ploeger for their constant interest and
versatile promotion. Thanks deserves an extra Nicolaas Busscher for the
long-term partnership.

Bernhard Johannes Kahl Witzenhausen, March 2006